Product Name
AKT (phospho T308) antibody
Catalog Number
GTX24796
Product Description:
Rabbit polyclonal to Akt (Phospho-Thr308)
Application Note:
WB: Use at a dilution of 1/500 - 1/1000. IHC-P: Use at a dilution of 1/50 - 1/100. Predicted molecular weight: 65 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tested Applications:
IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined.
Background:
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation
Clonality:
Polyclonal
Specificity:
Recognises endogenous levels of Akt only when phosphorylated at Threonine 308.
Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around the phosphorylation site of threonine 308
Host:
Rabbit
Isotype:
IgG
Cross Reactivity:
Human, mouse and rat - Not yet tested in other species
Positive Control:
Human lung carcinoma, extracts from 293 cells
Cellular Localization:
Cytoplasmic and Nuclear after activation by integrin linked protein kinase 1 (ILK1)
Purity:
Affinity purified with antigen
Purification Note:
The antibody was affinity-purified from rabbit antiserum by Affinity-chromatography using epitope-specific immunogen.
Storage Buffer:
Phosphate buffered saline, pH 7.3, 50% glycerol and 150mM NaCl containing 0.02% sodium azide
Storage Instruction:
Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
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