Product Name
14-3-3 beta antibody [4E1]
Catalog Number
GTX12341
Product Description:
Mouse monoclonal [4E1] to 14 - 3 - 3 beta
Application Note:
IP: Use at a concentration of 5 - 10 µg/ml. WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 29 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasma of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
Cross Reactivity:
Cross-reacts with Mouse and Rat. Not yet tested in other species.
Purity:
Protein A purified
Storage Instruction:
Store at 4 °C. Aliquot and store at -20 °C long-term.
Concentration:
1 mg/ml
Clone Number:
4E1
Storage Buffer:
Preservative: 1mg/ml Sodium AzideConstituents: PBS, 10mg/ml Sucrose. pH 7.4
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 beta
Clonality:
monoclonal
Lightchain:
kappa
Tested Applications:
Immunoprecipitation, Western blot
Positive Control:
Mouse brain tissue extract.
Isotype:
IgG2b
Immunogen:
Recombinant full length protein (Rat).
Host:
Mouse
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