Product Name
14-3-3 beta + epsilon + zeta antibody [3C8]
Catalog Number
GTX12346
Product Description:
Mouse monoclonal [3C8] to 14 - 3 - 3 beta + epsilon + zeta
Application Note:
WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
Cross Reactivity:
Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.
Purity:
Protein A purified
Storage Instruction:
Store at 4 °C. Aliquot and store at -20 °C long-term.
Concentration:
1 mg/ml
Clone Number:
3C8
Storage Buffer:
Preservative: None Constituents: 50% Glycerol, PBS. pH 7.2
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 beta + epsilon + zeta
Clonality:
monoclonal
Tested Applications:
Western blot
Positive Control:
HeLa Cell LysateMouse Brain Tissue ExtractRat Brain Tissue Extract
Isotype:
IgG2b
Specificity:
This antibody detects an ~30 kDa protein, corresponding to the apparent molecular mass of 14-3-3 beta, 14-3-3 epsilon or 14-3-3 zeta on SDS-PAGE immunoblots, in samples from human, mouse and rat origins.
Immunogen:
Human 14-3-3 beta fusion protein.
Host:
Mouse
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