Product Name
3T3 Lysate
Catalog Number
GTX27901
Product Description:
3T3 whole cell lysate
Application Note:
BALB/3T3 clone A31 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µgper lane is recommended for mini gel.
Background:
BALB/3T3 clone A31 cell lysate was prepared by homogenization in modified RIPA buffer(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetraacetic acid, 1 mM phenyl methyl sulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Tested Application:
Western blot
Purity:
Whole Cell Lysate
Storage Instruction:
Storage at -20 °C will be stable for three months, storage at-70 °C will be stable for one year.
Concentration:
2 mg/ml
Storage Buffer:
Whole lysate in 1 X SDS 5% b-mercaptoethanol
Target Protein:
3T3
Tested Applications:
Western blot
Form:
It is supplied as whole cell lysate, in 1 x SDS sample buffer containing 5% b-mercaptoethanol.
Concentration Unit:
mg/ml
Storage:
Storage at -20°C will be stable for three months, storage at-70°C will be stable for one year.
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