Product Name
RFX5
Catalog Number
GTX27544
Product Description:
RFX5 antibody panel ( anti - RFX5 C - term - N - term - internal )
Application Note:
For recommended dilutions, please see individual datasheets for each antibody. In western blotting a minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.
Background:
RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex, which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.
Cross Reactivity:
Human RFX5 from a variety of fibroblast and B-cell lysates.
Tested Application:
ELISA, Gel supershift assays, Immunoprecipitation, Western blot
Purity:
IgG fraction
Storage Instruction:
Store at 4 °C. Aliquot and store at -20 °C long-term.
Storage Buffer:
0.01M Na phosphate, 0.15M NaCl, pH7.4, 0.1% Na azide
Cross-Reactivity:
Human RFX5 from a variety of fibroblast and B-cell lysates.
Tested Applications:
ELISA, Gel supershift assays, Immunoprecipitation, Western blot
Specificity:
Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa.
Immunogen:
RFX5 (C-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH. (GTX26557)RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to KLH. (GTX27543)RFX5 peptide - aa 320 to 494 of the human protein conjugated to KLH. (GTX27542)
Storage:
Store at 4°C. Aliquot and store at -20°C long-term.
General References:
1. Steimle, V. et al., (1995) A novel DNA-binding regulatory factor is mutated in primary MHC class II deficiency (bare lymphocyte syndrome). Genes & Dev, 9(9):1021-32.
1995
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