Product Name
14-3-3 antibody
Catalog Number
GTX26081
Product Description:
Rabbit polyclonal to 14 - 3 - 3
Application Note:
IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 27-30k band and will cross react with alpha, beta, zeta, and theta isoforms, but is most strongly reactive with the Beta form. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Cross Reactivity:
Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.
Purity:
Immunogen affinity purified
Storage Instruction:
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Concentration:
0.2 mg/ml
Storage Buffer:
Preservative: 0.1% Sodium AzideConstituents: 0.2% Gelatin, PBS
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3
Clonality:
polyclonal
Tested Applications:
IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Form:
Liquid
Isotype:
IgG
Specificity:
Broadly reactive among the 14-3-3 family.
Immunogen:
Synthetic peptide mapping to the amino terminal domain of human 14-3-3.
Host:
Rabbit
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