Product Name
14-3-3 antibody [3F7]
Catalog Number
GTX14108
Product Description:
Mouse monoclonal [3F7] to 14 - 3 - 3
Application Note:
IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Cross Reactivity:
Cross-reacts with Human. Not yet tested in other species.
Purity:
Protein G purified
Storage Instruction:
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration:
batch dependent
Clone Number:
3F7
Storage Buffer:
Preservative: 0.09% Sodium AzideConstituents: 0.2% BSA, PBS. pH 7.4
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3
Clonality:
monoclonal
Lightchain:
kappa
Tested Applications:
Immunoprecipitation, Western blot
Positive Control:
HeLa whole cell lysate.
Isotype:
IgG1
Immunogen:
Recombinant full length protein (Human 14-3-3 gamma).
Host:
Mouse
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