Product Name
14-3-3 beta + zeta antibody
Catalog Number
GTX14113
Product Description:
Rabbit polyclonal to 14 - 3 - 3 beta + zeta
Manufacturer & Mfg Part#:
Abcam Ab14113
Application Note:
WB: Use at an assay dependent dilution. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
14-3-3 proteins are highly conserved proteins which play a role in both signal transduction and progression through the cell cycle by binding to and regulating several different proteins. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C. They mediate signal transduction by binding to phosphoserine-containing proteins. There are at least 7 mammalian isoforms: alpha, beta, gamma, delta, epsilon, zeta, and eta. An eighth subtype, termed theta has been found in rat brain. The 14-3-3 proteins exists in vitro and in vivo as either homo- or heterodimers which interact via their N-terminal domains and are subject to phosphorylation by protein kinase C. 14-3-3 proteins are localized in the cytoplasm of neurons in the cerebral cortex and are axonally transported to the nerve terminals. They may be present at lower levels in various other eukaryotic tissues. Northern blot analysis has shown expression of the eta chain in cultured cell lines derived from various tumors.
Cross Reactivity:
Cross-reacts with Human, all Mammals and Drosophilia melanogaster. Expected to cross-react with Xenopus laevis (100% identity with immunogen), Zebrafish (100% identity with immunogen) and Rainbow trout (91% identity with immunogen) due to sequence homology. Not yet tested in other species.
Purity:
Protein A purified
Concentration:
5 mg/ml
Storage Buffer:
Preservative: NoneConstituents: 30% Glycerol, PBS
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 beta + zeta
Clonality:
polyclonal
Tested Applications:
Western blot
Positive Control:
A431 whole cell lysate.
Isotype:
IgG
Specificity:
14-3-3 beta and Zeta isoforms.
Immunogen:
Synthetic peptide: ELVQKAKLAEQA (Human).
Host:
Rabbit
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