Product Name
14-3-3 sigma antibody [1.N.6]-BSA and Azide free
Catalog Number
GTX14116
Product Description:
Mouse monoclonal [1.N.6] to 14 - 3 - 3 sigma - BSA and Azide free
Application Note:
IHC-P: Use at a concentration of 2 - 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Cross Reactivity:
Cross-reacts with Human. Not yet tested in other species.
Purity:
Protein G purified
Concentration:
0.5 mg/ml
Clone Number:
1.N.6
Storage Buffer:
Preservative:None Constituents: PBS. pH 7.4
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 sigma
Clonality:
monoclonal
Lightchain:
kappa
Tested Applications:
IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Positive Control:
HT29 cell lysate and skin tissue slides.
Isotype:
IgG
Specificity:
Does not cross-react with other 14-3-3 isoforms.
Immunogen:
Recombinant full length protein (Human).
Host:
Mouse
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