Product Name
14-3-3 gamma antibody [3F8]
Catalog Number
GTX14118
Product Description:
Mouse monoclonal [3F8] to 14 - 3 - 3 gamma
Application Note:
IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 proteins regulate many cellular processes that are important in cancer biology, such as apoptosis and cell cycle checkpoints. Evidence indicates 14-3-3 is required for Raf1 kinase activity and phosphorylation amoung many other functions. Levels of 14-3-3 gamma in the brain are significantly increased in Alzheimer's disease and Down syndrome.
Cross Reactivity:
Cross-reacts with Human. Not yet tested in other species.
Purity:
Protein G purified
Concentration:
1 mg/ml
Clone Number:
3F8
Storage Buffer:
Preservative: NoneConstituents: 10mM PBS
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 gamma
Clonality:
monoclonal
Lightchain:
kappa
Tested Applications:
Immunoprecipitation, Western blot
Positive Control:
HeLa whole cell lysate.
Isotype:
IgG1
Specificity:
No crossreactivity with other isoforms of 14-3-3.
Immunogen:
Recombinant full length protein (Human 14-3-3 gamma).
Host:
Mouse
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