Product Name
14-3-3 antibody [2Q248]
Catalog Number
GTX14121
Product Description:
Mouse monoclonal [2Q248] to 14 - 3 - 3
Application Note:
WB: Use at a dilution of 1/50 - 1/500, incubate for 2 hours at 23°C with alkaline phosphatase secondary antibody and BCIP/NBT color development. Detects a band of approximately 33 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Cross Reactivity:
Cross-reacts with Rat. Not yet tested in other species.
Purity:
Ascites
Clone Number:
2Q248
Storage Buffer:
Supplied as a liqud in 0.1% sodium azide, before the addition of glycerol to 40%.
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3
Clonality:
monoclonal
Tested Applications:
Western blot
Isotype:
IgG2a
Specificity:
Detection varies from tissue to tissue (Brain, Retina, Testis, Pineal, Kidney, Spleen, Adrenal, Lung, Heart, Liver, Ovary, Thyroid) and during development. This is a pan specific 14-3-3 antibody.
Immunogen:
Synthetic peptide, corresponding to amino acids 208-244 of Rat 14-3-3.
Host:
Mouse
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