Product Name
14-3-3 sigma antibody [1.T.28]
Catalog Number
GTX14122
Product Description:
Mouse monoclonal [1.T.28] to 14 - 3 - 3 sigma
Application Note:
WB: Use at a concentration of 0.5 µg/ml. GTX14122 detects 20µg of reduced EGF-stimulated A431 cell lysate, visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Cross Reactivity:
Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.
Purity:
Protein G purified
Concentration:
Batch dependent
Clone Number:
1.T.28
Storage Buffer:
Preservative: 0.05% Sodium AzideConstituents: 30% Glycerol, 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 sigma
Clonality:
monoclonal
Tested Applications:
Western blot
Positive Control:
EGF-stimulated A431 cell lysate.
Form:
Liquid
Isotype:
IgG1
Specificity:
GTX14122 recognizes 14-3-3 sigma and does not crossreact with other 14-3-3 isoforms.
Immunogen:
Fusion protein corresponding to full-length human 14-3-3 sigma.
Host:
Mouse
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