Product Name
14-3-3 sigma antibody [1.N.6]
Catalog Number
GTX14123
Product Description:
Mouse monoclonal [1.N.6] to 14 - 3 - 3 sigma
Application Note:
IF: Use at an assay dependent dilution. IHC-P: Use at a concentration of 2 - 4 µg/ml for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 minutesIP: Use 2µg/mg protein lysate. Use protein G. WB: Use at a concentration of 1 µg/ml for 2 hours at room temperature. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf-1 kinase activity and phosphorylation amoung many other functions.
Cross Reactivity:
Cross-reacts with Human. Not yet tested in other species.
Purity:
Protein G purified
Concentration:
0.1 mg/ml
Clone Number:
1.N.6
Storage Buffer:
Preservative: 0.09% Sodium AzideConstituents: 40% Glycerol, 0.2% BSA, 10mM PBS. pH 7.4
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 sigma
Clonality:
monoclonal
Lightchain:
kappa
Tested Applications:
Immunofluorescence, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Positive Control:
HT29 cells and skin
Isotype:
IgG1
Specificity:
Recognizes human 14-3-3 sigma protein, 26-30kD. Does not crossreact with other isoforms of 14-3-3.
Immunogen:
Recombinant full length protein (Human).
Host:
Mouse
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