Product Name
14-3-3 Binding Motif (phospho S) antibody [3i2]
Catalog Number
GTX14126
Product Description:
Mouse monoclonal [3i2] to 14 - 3 - 3 Binding Motif ( phospho S )
Application Note:
ELISA: Use at a dilution of 1/1000. IP: Use at a dilution of 1/20 on denatured proteins. WB: Use at a dilution of 1/4000. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.
Cross Reactivity:
Recognises the phospho 14-3-3 binding motif.
Purity:
Protein A purified
Clone Number:
3i2
Storage Buffer:
Preservative: 0.02% sodium azide; Constituents: 50% Glycerol, BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5
Cellular Localization:
Cytoplasmic
Target Protein:
14-3-3 Binding Motif
Clonality:
monoclonal
Tested Applications:
ELISA, Immunoprecipitation, Western blot
Isotype:
IgG1
Specificity:
GTX14126 binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA the antibody recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. The antibody weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences.
Immunogen:
Synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled).
Host:
Mouse
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