Product Name
ASK1 antibody
Catalog Number
GTX14379
Product Description:
Rabbit polyclonal to ASK1
Application Note:
WB: Use at a concentration of 1:500-1:1000. Predicted molecular weight: 155 kDa. IHC-P: Use at a concentration of 1:50-1:100. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tested Applications:
IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined.
Background:
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF-a induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Clonality:
Polyclonal
Immunogen:
Synthesized non-phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83
Host:
Rabbit
Isotype:
IgG
Cross Reactivity:
Human - Not yet tested in other species - Expected to cross-react with Mouse (93% identity with immunogen) due to sequence homology
Positive Control:
RIPA lysates from Human SW1353 cell
Target Protein:
ASK1
Form:
Liquid
Concentration:
1 mg/ml
Purity:
Affinity purified with antigen
Purification Note:
From polyclonal serum
Storage Buffer:
Phosphate buffered saline, pH 7.3, 50% glycerol and 150mM NaCl containing 0.02% sodium azide
Storage Instruction:
Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
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