Product Name
ASK1 phospho S83 antibody
Catalog Number
GTX14380
Product Description:
Rabbit polyclonal to ASK1 (phospho S83)
Application Note:
For WB: Use at a dilution of 1:500-1:1000. Predicted molecular weight: 155 kDa. For IHC-P: Use at a dilution of 1:50-1:1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tested Application:
IHC (Formalin-fixed paraffin-embedded sections), Western blot. The usefulness of this product in other applications has not been determined.
Background:
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activate c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induces apoptotic cell death, and a catalytically inactive form of ASK1 inhibits TNF-a induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Clonality:
Polyclonal
Specificity:
ASK1 (phospho-Ser83) antibody detects endogenous levels of ASK1 only when phosphorylated at serine 83.
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83 (G-S-S^P-V-G).
Host:
Rabbit
Isotype:
IgG
Cross Reactivity:
Human - Not yet tested in other species
Target Protein:
ASK1
Cellular Localization:
Cytoplasmic
Form:
Liquid
Concentration:
1 mg/ml
Purity:
Affinity purified with antigen
Purification Note:
From polyclonal serum
Storage Buffer:
Phosphate buffered saline, pH 7.3, 50% glycerol and 150mM NaCl containing 0.02% sodium azide
Storage:
Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
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