Product Name
Acrp30 protein (His tag)
Catalog Number
GTX13882
Product Description:
Acrp30 protein (His tag)
Application Note:
ELISA: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
The Human Adiponectin is created as a recombinant protein with N-terminal fusion of HisTag. The Human Adiponectin His-Tagged Fusion Protein, produced in E. coli, is 26.4 kDa protein containing 230 amino acid residues of the human Adiponectin and 12 additional amino acid residues - HisTag
Immunogen:
Recombinant full length protein: MRGSHHHHHH GSGHDQETTT QGPGVLLPLP KGACTGWMAG IPGHPGHNGA PGRDGRDGTP GEKGEKGDPG LIGPKGDIGE TGVPGAEGPR GFPGIQGRKG EPGEGAYVYR SAFSVGLETY (Human).
Cross Reactivity:
Cross-reacts with Human.The amino acid sequence of the recombinant human adiponectin is 100% homologous to the amino acid sequence of the human serum adiponectin. Not yet tested in other species.
Tested Applications:
ELISA, Western blot
Target Protein:
Acrp30
Cellular Localization:
Secreted
Purity:
>95% by SDS-PAGE
Purification Note:
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Storage Buffer:
Sterile filtered and lyophilized from 0.5 mg/ml in 0.02M Tris buffer pH 7.5, 0.15M NaCl.
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