Product Name
EL4 lysate
Catalog Number
GTX27183
Product Description:
EL4 whole cell lysate
Application Note:
EL4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
Background:
Mouse EL4 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Purity:
Whole Cell Lysate
Concentration:
2 mg/ml
Storage Buffer:
Whole cell lysate in 1 X SDS-PAGE sample loading buffer, with 5% b-mercaptoethanol
Target Protein:
EL4
Tested Applications:
Western blot
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