Product Name
A431 Lysate EGF treated
Catalog Number
GTX14635
Product Description:
A431 Lysate EGF treated
Application Note:
Add 2.5µl of 2-mercaptoethanol/100µl of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for immunoblot analysis. This preparation may be used as a positive control for antibodies. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor. A431 cells were cultured for 20 minutes in the presence of 50ng/ml EGF. Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1µg/ml aprotinin, 1µg/ml leupeptin, 1µg/ml pepstain, 1mM sodium fluoride) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% brophenol blue).
Purity:
Whole Cell Lysate
Storage Instruction:
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Concentration:
1 mg/ml
Target Protein:
A431
Tested Applications:
The usefulness of this product in other applications has not been determined.
Form:
Liquid
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