Product Name
AtT-20/D16v-F2 Nuclear Extract
Catalog Number
GTX14636
Product Description:
AtT-20/D16v-F2 Nuclear Extract
Application Note:
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Background:
Clone AtT-20, an ACTH secreting cell line, was cloned in October, 1966, by G. Sato and associates from earlier cultures established after alternate passage of mouse pituitary tumor cells as tumors in animals and in cell culture. The mouse pituitary tumor was originally established in LAF1 mice by J. Furth, et al.
GTX14636 AtT-20/D16v-F2 nuclear extract is prepared from AtT-20/D16v-F2 cells, a subclone of AtT-20, developed by B. Gumbiner.
Storage Instruction:
Stored at -80°C.
For best stability, aliquot the extracts into single-use fractions and store at -80°C to avoid repeated freeze/thaw cycles.
Storage Buffer:
Preservative: None.
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
Tested Applications:
Electrophoretic mobility shift assay, Western blot
Form:
Liquid
Host:
Mouse
General References:
1. Furth J , et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953.
1953
13121002
2. Gumbiner B , Kelly RB . Two distinct intracellular pathways transport secretory and membrane glycoproteins to the surface of pituitary tumor cells. Cell 28: 51-59, 1982.
1982
6279313
3. Moore HP , et al. Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation. Cell 35: 531-538, 1983.
1983
6317196
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