Product Name
COS-7 CoCl2 Treated Lysate
Catalog Number
GTX14644
Product Description:
COS - 7 CoCl2 Treated Lysate
Application Note:
Each lot has been tested for HIF activation. The signal intensity for HIF activation in each lot is compared to the signal intensity obtained with extracts from unstimulated COS-7 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of HIF activation in a given cell type. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Purity:
Nuclear Lysate
Concentration:
2.5 mg/ml
Storage Buffer:
Preservative: NoneConstituents (lysis buffer): 5% Beta-mercaptoethanol, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10mM PNPP, 10 mM beta-glycerophosphate, 20% Glycerol, 1 x SDS, 0.1M EDTA, 400mM Sodium Chloride, 20mM HEPES, 1mM DTT and protease inhibitors. pH 7.5
Target Protein:
COS-7
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