Product Name
Pronase Reagent
Catalog Number
GTX73181
Product Description:
Pronase Reagent
Application Note:
For IHC: Use as antigen retriever for some antibodies in IHC. Reagent is supplied in ready-to-use format. Over-digestion may detach tissue sections from slides or may result in the loss of tissue morphology. It may be necessary to use + charge, sialanized or polylysine coated slides.
Procedure:
1. Deparafinize FFPE tissue section is carried out as outlined in your protocol. Hydrate tissue with PBS or Immuno buffer.
2. Remove buffer, add 2-4 drops of Pronase reagent to cover tissue section.
3. Incubate at room temp. for 5 minutes (the time required for optimum digestion of FFPE tissue section will vary with the extent of fixation, generally approx. 5 minutes are sufficient at room temp. In some cases it may require 15 minutes at room temp).
4. Discard Pronase reagent, wash 3-5 times with buffer.
5. The tissue sections are ready for further IHC protocol.
Background:
Pronase is used for photolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage Instruction:
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Tested Applications:
Immunohistochemistry. The usefulness of this product in other applications has not been determined.
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