Product Name
HRP Substrate-Chromogen (DAB Tablets) Kit
Catalog Number
GTX73327
Product Description:
DAB (Diaminobenzidine) chromogen
Application Note:
See provided protocol for suggestions.
Background:
These products are designed to produce a color deposition on tissue sections processed by immunocytochemical techniques using peroxidase as tracers. When using these products, it is very important to select the appropriate chromogen substrate for the enzyme tracer being used in the assay.
One mL of final working solution will yield approximately 5-6 tissue sections. The number of tests performed will vary depending on the application. Estimations are derived from use with the capillary-action based MicroProbe¿ system, which draws 150-200 mL per test. Each 20-tablet kit contains sufficient reagents to make 100 mL of final working solution. The 100 tablet chromogen-only kit will yield 500 mL of final working solution. All other kits contain sufficient reagents to make 300 mL of final working solution.
Storage Instruction:
Store at 4°C. Do not freeze.
Tested Applications:
Immunohistochemistry, Western blot. The usefulness of this product in other applications has not been determined.
Form:
This product is supplied as:
3A: Conc. buffer 5mls
3B: Chromogen tablets 20
3C: Conc. substrate 1ml
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