Product Name
Bad phospho S112 antibody
Catalog Number
GTX21435
Product Description:
Rabbit polyclonal to Bad ( phospho S112 )
Application Note:
IP: Use at a concentration of 10 µg/ml. WB: Use at a concentration of 1.0 µg/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tested Application:
Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Background:
Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform
Clonality:
Polyclonal
Specificity:
The antibody detects Bad only when phosphorylated at Ser 112.
Host:
Rabbit
Isotype:
IgG
Cross Reactivity:
Human, Mouse and Rat - Not yet tested in other species
Target Protein:
Bad
Cellular Localization:
Mitochondrial
Form:
Liquid
Concentration:
1 mg/ml
Purity:
Affinity purified with antigen
Storage Buffer:
Phosphate buffered saline, pH 7.3, 50% glycerol and 150mM NaCl containing 0.02% sodium azide
Storage:
Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Specific References:
1. Yang E et al. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell 80:285-91 (1995).
1995
2. Zha J et al. Serine phosphorylation of death agonist BAD in response to survival factor results in binding to 14-3-3 not BCL-X(L) Cell 87:619-28 (1996).
1996
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