Product Name
Affinity Purified Chicken anti-Rabbit IgG h+l Absorbed Against Human and Mouse IgG [HRP]
Catalog Number
GTX77914
Product Description:
Affinity Purified Chicken anti - Rabbit IgG h+l Absorbed Against Human and Mouse IgG (HRP-conjugated)
Application Note:
Western blot - Use at 1:1,000 to 1:5,000 dilution. ELISA - Recommended starting diultion of 1:10,000. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Background:
The affinity purified anti rabbit IgG reacts with rabbit immunoglobulins and the papain derived Fc and Fab fragments by IEP and gel diffusion techniques. There is no reaction with mouse or human serum. Chickens were immunized with highly purified rabbit IgG isolated from normal pooled rabbit serum, in Freund's adjuvant. Eggs were collected, the IgY fraction prepared and the anti rabbit IgG immunoaffinity purified off a solid phase immunoabsorbant. Anti Rabbit IgG was further absorbed against mouse and human IgG. The affinity-purified antibody was conjugated with horseradish peroxidase.
Specificity:
Rabbit
Cross Reactivity:
Rabbit
Tested Applications:
ELISA, Immunoelectrophoresis, Western blot. The usefulness of this product in other applications has not been determined.
Host:
Chicken
Clonality:
Polyclonal
Species secondary against:
Rabbit
Conjugation:
Horse Radish Peroxidase
Form:
Liquid
Concentration:
1 mg/ml
Storage Buffer:
0.01M Phosphate Buffered Saline, 0.025% sodium azide
Storage Instruction:
Store at 4 °C.
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