Product Name
26S Proteasome Fraction Extract
Catalog Number
GTX80879
Product Description:
26S Proteasome Fraction Extract
Background:
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman¿s syndrome, and Liddle syndrome.
GTX80879 is the partially purified fraction containing 26S proteasome and none of the conjugation enzymes (E1, E2s, E3s). Preferred for the degradation of pre-formed ubiquitin-protein conjugates by the 26S proteasome since highly purified 26S is costly and more labile.
Form:
0.5 mg/ml in 50 mM HEPES, pH 7.6, 1 mM Mg-ATP, 150 mM NaCl and 10% glycerol.
Storage:
-80º C, Avoid Freeze/Thaw Cycles
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