Product Name
CD18 antibody [CLB-LFA-1/1, 54]
Catalog Number
GTX00001
Product Description:
Mouse monoclonal [CLB-LFA-1/1, 54] to CD18
Application Note:
Flow Cyt: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Tested Application:
Flow cytometry/FACS, Immunofluorescence. The usefulness of this product in other applications has not been determined.
Background:
CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
Clonality:
Monoclonal
Clone Number:
CLB-LFA-1/1, 54
Specificity:
GTX00001 is directed against the CD18 antigen (Lymphocyte Function-associated Antigen-1, integrin ß2 chain), which is expressed on mature immunocompetent lymphocytes and their neoplastic counterparts, granulocytes and monocytes. It is absent in patients with LAD-1.
Immunogen:
Tissue / cell preparation: patient cells
Host:
Mouse
Myeloma:
Mouse SP2/0 myeloma line.
Isotype:
IgG1
Cross-Reactivity:
Reacts with Human. Not yet tested in other species.
Cellular Localization:
Membrane; single pass type I membrane protein.
Form:
Liquid
Concentration:
0.2
Concentration Unit:
mg/ml
Purity:
Ion Exchange Chromatography
Purification Note:
GTX00001 was purified from ascites by ammonium sulphate precipitation and ion exchange chromatography.
Storage Buffer:
Preservative: 0.001% Thimerosal (merthiolate); Constituents: 10mg/ml BSA, 20mM Tris, 150mM Sodium chloride, pH 8
Storage:
Store at +4°C in the dark.
General References:
1. Miedema F et al. Immunologic studies with LFA-1- and Mo1-deficient lymphocytes from a patient with recurrent bacterial infections. J Immunol 134:3075-81 (1985).
1985
3156928
2. Miedema F et al. Both Fc receptors and lymphocyte-function-associated antigen 1 on human T gamma lymphocytes are required for antibody-dependent cellular cytotoxicity (killer cell activity). Eur J Immunol 14:518-23 (1984).
1984
6610556
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