Product Name
Angiopoietin 1 antibody
Catalog Number
GTX28451
Product Description:
Rabbit polyclonal to Angiopoietin 1
Application Note:
Western blot 1/500There is reaction with serum in the cell supernatants, which results in strongbackground and makes it difficult to see the angiopoietins in cellsupernatants. However when precipitated (using soluble Tie2) the signals are very good and strong.
Background:
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognizes a band above 55 kD. This antibody (and the ang-2 antibody, GT28452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher that the calculated molecular weight of 55 kD.
Tested Application:
Western blot
Cross Reactivity:
Cross-reacts with Mouse. Not yet tested in other species.
Purity:
Whole antiserum
Storage Instruction:
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for
Concentration:
85 mg/ml
Storage Buffer:
0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2
Cross-Reactivity:
Mouse - specific for mAng-1. This antibody does recognise human angiopoietin-1 although there seems to be some reactivity towards human Ang-2.
Target Protein:
Angiopoietin 1
Clonality:
polyclonal
Tested Applications:
Western blot
Form:
Liquid (sterile filtered) Protein Concentration: 85 mg/ml (by Refractometry)
Isotype:
IgG
Specificity:
This antibody is specific for mouse Angiopoietin 1 (mAng-1), and shows a band at 75kD.
Immunogen:
Peptide: CNQRRNPENGGRRYNRIQHGQ (available as GTX29076)Region near the N-terminus of mouse Ang-1 (aa21-40)
Host:
Rabbit
Storage:
Store vial at -20 ° C prior to opening. Aliquot contents and freeze at -20 ° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
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