Product Name
ATM [2C1] antibody
Catalog Number
GTX70103
Product Description:
Mouse monoclonal [2C1] to ATM
Manufacturer & Mfg Part#:
GeneTex GTX70103
Application Note:
For WB: Use at a dilution of 1:500-1:1,000. For IP: Use at a concentration of 1-10 µg/ml. Immunohistochemistry analyses was performed of the cerebral cortex of E14.5 wild-type (+/+) and scid mice - (MC Vemuri, 2001) For immunofluorescence please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Background:
ATM-2C1 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.
Tested Application:
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Positive Control:
Raji and lymphoblastoid nuclear lysate
Target Protein:
ATM
Target Protein MW:
370kD
Clonality:
Monoclonal
Clone Number:
2C1
Purity:
Protein G purified
Purification Note:
From Ascites
Concentration:
1 mg/ml
Form:
Liquid
Storage Buffer:
Phosphate-buffered saline, pH7.4, containing no preservatives
Cross-Reactivity:
Human, Rat, Mouse, and Monkey - Not yet tested in other species
Expression system:
pMAL
Myeloma:
NS1
Isotype:
IgG1
Lightchain:
Kappa
Cellular Localization:
Nucleus
Immunogen:
Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
Host:
Mouse
Storage:
Keep as concentrated solution. Aliquot and store at -20şC or below. Avoid freeze-thaw cycles.
General References:
1. Chen, G. and Lee, E.Y-H.P. (1996) The product of the ATM gene is a 370-kDa nuclear phosphoprotein. J Biol Chem 271: 33693-33697.
1996
8969240
2. Suzuki, K., Kodama, S. and Watanabe, M. (1999) Recruitment of ATM protein to double strand DNA irradiated with ionizing radiation. J Biol Chem 274: 25571-25575.
1999
Specific References:
1. Harry Scherthan, Martin Jerratsch, Sonu Dhar, Y. Alan Wang, Stephen P. Goff, and Tej K. Pandita. Meiotic Telomere Distribution and Sertoli Cell Nuclear Architecture Are Altered in Atm- and Atm-p53-Deficient Mice. Mol. Cell. Biol., Oct 2000; 20: 7773 - 7783.
2000
11003672
2. Gang Chen and Eva Y.-H. P. Lee. The Product of the ATM Gene Is a 370-kDa Nuclear Phosphoprotein. J. Biol. Chem., Dec 1996; 271: 33693 - 33697.
1996
8969240
3. Heather Beamish, Padmini Kedar, Hideo Kaneko, Philip Chen, Toshiyuki Fukao, Cheng Peng, Sergei Beresten, Nuri Gueven, David Purdie, Susan Lees-Miller, Nathan Ellis, Naomi Kondo, and Martin F. Lavin. Functional Link between BLM Defective in Bloom's Syndrome and the Ataxia-telangiectasia-mutated Protein, ATM. J. Biol. Chem., Aug 2002; 277: 30515 - 30523.
2002
4. Yi Wang, David Cortez, Parvin Yazdi, Norma Neff, Stephen J. Elledge, and Jun Qin . BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures. Genes & Dev., Apr 2000; 14: 927 - 939.
2000
5. Thilo Dörk, Regina Bendix, Michael Bremer, Dirk Rades, Karin Klöpper, Marion Nicke, Britta Skawran, Ariadne Hector, Paria Yamini, Diana Steinmann, Sabine Weise, Manfred Stuhrmann, and Johann H. Karstens . Spectrum of ATM Gene Mutations in a Hospital-based Series of Unselected Breast Cancer Patients. Cancer Res., Oct 2001; 61: 7608 - 7615.
2001
6. Paul R. Borghesani, Frederick W. Alt, Andrea Bottaro, Laurie Davidson, Saime Aksoy, Gary A. Rathbun, Thomas M. Roberts, Wojciech Swat, Rosalind A. Segal, and Yansong Gu . Abnormal development of Purkinje cells and lymphocytes in Atm mutant mice. PNAS, Mar 2000; 97: 3336 - 3341.
2000
7. Michael M Weil, Frances S Kittrell, Yongjia Yu, Maureen McCarthy, Ryan C Zabriskie, Robert L Ullrich. Radiation induces genomic instability and mammary ductal dysplasia in Atm heterozygous mice. Oncogene 20, 4409 - 4411 (17 Jul 2001)
2001
8. Shang Li, Nicholas S. Y. Ting, Lei Zheng, Phang-Lang Chen, Yael Ziv, Yosef Shiloh, Eva Y.-H. P. Lee, Wen-Hwa Lee. Functional link of BRCA1 and ataxia telangiectasia gene product in DNA damage response. Nature 406, 210 - 215 (13 Jul 2000)
2000
9. M C Vemuri, E Schiller, J R Naegele. Elevated DNA double strand breaks and apoptosis in the CNS of scid mutant mice. Cell Death and Differentiation 8, 245 - 255 (22 Mar 2001)
2001
10. Lubomir B. Smilenov, David J. Brenner, and Eric J. Hall . Modest Increased Sensitivity to Radiation Oncogenesis in ATM Heterozygous versus Wild-Type Mammalian Cells. Cancer Res., Aug 2001; 61: 5710 - 5713.
2001
11. Baocheng Hu, Xiang-Yang Zhou, Xiang Wang, Zhao-Chong Zeng, George Iliakis, and Ya Wang. The Radioresistance to Killing of A1-5 Cells Derives from Activation of the Chk1 Pathway. J. Biol. Chem., May 2001; 276: 17693 - 17698.
2001
12. Wu X, Shell SM, Liu Y, Zou Y. ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation. Feb 1;26(5):757-64.
2007
16862173
13. Microcephalin Is a DNA Damage Response Protein Involved in Regulation of CHK1 and BRCA1. Xingzhi Xu, Juhie Lee, and David F. Stern. J. Biol. Chem. 2004 279(33): p. 34091-34094
2004
14. M L Cortés, C J Bakkenist, M V Di Maria, M B Kastan, X O Breakefield. HSV-1 amplicon vector-mediated expression of ATM cDNA and correction of the ataxia?telangiectasia cellular phenotype. Gene Therapy 10, 1321 - 1327 (01 Aug 2003)
2003
15. Sergei Kozlov, Nuri Gueven, Katherine Keating, Jonathan Ramsay, and Martin F. Lavin. ATP Activates Ataxia-Telangiectasia Mutated (ATM) in Vitro. IMPORTANCE OF AUTOPHOSPHORYLATION. J. Biol. Chem., Mar 2003; 278: 9309 - 9317.
2003
16. A. Fedier, M. Schlamminger, V. A. Schwarz, U. Haller, S. B. Howell, and D. Fink. Loss of atm sensitises p53-deficient cells to topoisomerase poisons and antimetabolites. Ann. Onc., Jun 2003; 14: 938 - 945.
2003
17. Jong-Soo Kim, Tatiana B. Krasieva, Vickie LaMorte, A. Malcolm R. Taylor, and Kyoko Yokomori. Specific Recruitment of Human Cohesin to Laser-induced DNA Damage. J. Biol. Chem., Nov 2002; 277: 45149 - 45153.
2002
18. Eric J. Perkins, Ayyappan Nair, Dale O. Cowley, Terry Van Dyke, Yung Chang, and Dale A. Ramsden. Sensing of intermediates in V(D)J recombination by ATM. Genes & Dev., Jan 2002; 16: 159 - 164.
2002
19. Pierre-Marie Girard, Enriqueta Riballo, Adrian C Begg, Alastair Waugh, Penny A Jeggo. Nbs1 promotes ATM dependent phosphorylation events including those required for G1/S arrest. Oncogene 21, 4191 - 4199 (20 Jun 2002)
2002
20. Shaun P. Scott, Regina Bendix, Philip Chen, Raymond Clark, Thilo Dörk, and Martin F. Lavin . Missense mutations but not allelic variants alter the function of ATM by dominant interference in patients with breast cancer. PNAS, Jan 2002; 99: 925 - 930.
2002
21. Ryoko Araki, Ryutaro Fukumura, Akira Fujimori, Yoichi Taya, Yosef Shiloh, Akihiro Kurimasa, Sandeep Burma, Gloria C. Li, David J. Chen, Koki Sato, Yuko Hoki, Kouichi Tatsumi, and Masumi Abe . Enhanced Phosphorylation of p53 Serine 18 following DNA Damage in DNA-dependent Protein Kinase Catalytic Subunit-deficient Cells. Cancer Res., Aug 1999; 59: 3543 - 3546.
1999
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