Product Name
ATR [2B5] antibody
Catalog Number
GTX70109
Product Description:
Mouse monoclonal [2B5] to ATR
Manufacturer & Mfg Part#:
GeneTex GTX70109
Application Note:
For WB: Use at a dilution of 1:500-1,000. For IP and IF information please refer to publications. Not yet tested in other applications. Optimal dilution should be determind by the researcher.
Background:
Ataxia Telangiectasia Mutated (ATM) and Rad3-related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.
Target Protein MW:
317
Purity:
Protein G purified
Myeloma:
NS1
Concentration:
1 mg/ml
Clone Number:
2B5
Storage Buffer:
Phosphate-buffered saline, pH7.2
Cross-Reactivity:
Human - Not yet tested in other species
Target Protein:
ATR
Purification Note:
From Ascites
Clonality:
Monoclonal
Tested Applications:
Immunofluorescence, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Positive Control:
Raji whole cell lysate
Form:
Liquid
Isotype:
IgG
Immunogen:
Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.
Host:
Mouse
Storage:
Aliquot and store at -20 to -80 °C
General References:
1. Abraham RT Cell cycle checkpoint signaling through the ATM and ATR kinases. Genes Dev 15:2177-96 (2001).
2001
11544175
2. Bradbury JM & Jackson SP ATM and ATR. Curr Biol 13:R468 (2003).
2003
12814562
Specific References:
1. Wu X, Shell SM, Liu Y, Zou Y. ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation. Feb 1;26(5):757-64.
2007
16862173
2. Sean Post, Yi-Chinn Weng, Karlene Cimprich, Lan Bo Chen, Yang Xu, and Eva Y.-H. P. Lee. Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G1/S checkpoint activation in response to DNA damage. PNAS, Nov 2001; 98: 13102 - 13107.
2001
3. Julie K. Horton, Donna F. Stefanick, Jana M. Naron, Padmini S. Kedar, and Samuel H. Wilson. Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest following DNA methylating agent exposure. February 2005, 10.1074/jbc.M413841200
2005
4. Jia Li and David F. Stern. Regulation of Chk2 by DNA-dependent protein kinase. J. Biol. Chem. published 24 January 2005, 10.1074/jbc.M412445200
2005
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