GTX27963 staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)
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c-fos antibody (GTX27963) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal cord (not shown). Staining performed in free floating IHC on 30µm saggital spinal cord sections (perfusion fixed tissue). Find detailed info regarding the protocol in the Specific Protocol link. Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
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c-fos antibody (GTX27963) at a dilution of 1/500 (1 day incubation at RT; secondary antibody Alexa fluor 488 at 1/1000). Nuclear staining observed in the rat spinal cord dorsal horn [A] X10 and [B] X20 magnification. Immunofluorescence staining performed on slide on 30µm saggital sections of spinal cord (PFA/picric acid perfusion fixed tissue; 2 days tissue post-fixation). Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
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Product Name
c-Fos antibody
Catalog Number
GTX27963
Product Description:
Rabbit polyclonal to c-Fos
Manufacturer & Mfg Part#:
Abcam AB7963
Application Note:
For IHC-P: Use at a dilution of 1:1,000. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Background:
The products of the proto-oncogenes c-Jun (c-Jun was previously known as the Fos-binding protein p39) and c-Fos are known to form a complex in the nucleus (2, 6). AP-1 (activating protein-1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF (activating transcription factor) subunits that bind to a common DNA site, the AP-1-binding site (1). There is evidence that AP-1 proteins, mostly those that belong to the Jun group, control cell life and death through their ability to regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16 (3). The Fos and Jun proto-oncogenes expression is induced transiently by a great variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons (5). Studies have established a unique role for Fos in determining the differentiation and activity of progenitors of the osteoclast lineage (4).
Purity:
Affinity purified with antigen
Concentration:
0.2 mg/ml
Storage Buffer:
Phosphate-buffered saline containing 0.1% sodium azide and 0.2%gelatin
Cross-Reactivity:
Human, Mouse, and Rat - Not yet tested in other species
Target Protein:
c-Fos
Clonality:
Polyclonal
Tested Applications:
IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Positive Control:
A-431 and HeLa lysate
Form:
Liquid
Isotype:
IgG
Specificity:
Reacts with c-Fos
Immunogen:
Synthetic peptide (Human) (N terminal).
Host:
Rabbit
Storage:
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
General References:
1. Karin M, Liu Zg, Zandi E. AP-1 function and regulation. Curr Opin Cell Biol. 1997 Apr;9(2):240-6.
1997
2. Bos TJ, Rauscher FJ 3rd, Curran T, Vogt PK. The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein. Oncogene. 1989 Feb;4(2):123-6.
1989
3. Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001 Apr 30;20(19):2390-400.
2001
4. Liebermann DA, Gregory B, Hoffman B. AP-1 (Fos/Jun) transcription factors in hematopoietic differentiation and apoptosis. Int J Oncol. 1998 Mar;12(3):685-700.
1998
5. Curran T. Fos and Jun: oncogenic transcription factors. Tohoku J Exp Med. 1992 Oct;168(2):169-74.
1992
6. Rauscher FJ 3rd, Voulalas PJ, Franza BR Jr, Curran T. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro. Genes Dev. 1988 Dec;2(12B):1687-99.
1988
7. Karin M, Liu Zg, Zandi E. AP-1 function and regulation. Curr Opin Cell Biol. 1997 Apr;9(2):240-6.
1997
8. Bos TJ, Rauscher FJ 3rd, Curran T, Vogt PK. The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein. Oncogene. 1989 Feb;4(2):123-6.
1989
9. Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001 Apr 30;20(19):2390-400.
2001
10. Liebermann DA, Gregory B, Hoffman B. AP-1 (Fos/Jun) transcription factors in hematopoietic differentiation and apoptosis. Int J Oncol. 1998 Mar;12(3):685-700.
1998
11. Curran T. Fos and Jun: oncogenic transcription factors. Tohoku J Exp Med. 1992 Oct;168(2):169-74.
1992
12. Rauscher FJ 3rd, Voulalas PJ, Franza BR Jr, Curran T. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro. Genes Dev. 1988 Dec;2(12B):1687-99.
1988
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