Product Name
TRIP230 [10G5] antibody
Catalog Number
GTX70253
Product Description:
Mouse monoclonal antibody to human TRIP230
Manufacturer & Mfg Part#:
GeneTex GTX70253
Application Note:
For WB: Use at a dilution of 1:500. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Background:
The Trip230 gene encodes a protein of 1,978 amino acids with an estimated molecular mass of 230 kDa. The gene is located on chromosome 14q31, which is in a region associated with genetic alterations and thyroid hormone response abnormalities. The Trip230 protein interacts with Rb and thyroid hormone receptor (TR) using two distinct domains. Complex formation with Rb is in a hormone independent manner, but when Trip230 forms a complex with TR, it is in a thyroid hormone-dependent manner. After T3 treatment, Trip230 is phosphorylated and transported to the nucleus, suggesting that phosphorylation may be important for its nuclear translocation.
Target Protein MW:
230
Purity:
Protein G Affinity Purified
Expression system:
pGEX
Myeloma:
NS1
Concentration:
1 mg/ml
Clone Number:
10G5
Storage Buffer:
Phosphate-buffered saline, pH 7.4, containing no preservatives
Cross-Reactivity:
Human - Not yet tested in other species
Target Protein:
TRIP230
Purification Note:
From hybridoma tissue culture supernatant
Clonality:
Monoclonal
Lightchain:
Kappa
Tested Applications:
Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.
Positive Control:
MCF7, HBL100, T24
Form:
Liquid
Isotype:
IgG1
Immunogen:
Amino acids 1099-1372 of Trip230 expressed in E. coli.
Host:
Mouse
Storage:
Store at -80 °C. Avoid repeat freeze-thaw cycles.
General References:
1. Chen, Y., Chen, P.L., Chen, C.F., Sharp, Z.D., Lee, W-H. (1999) Thyroid hormone, T3-dependent phosphorylation and translocation of Trip230 from the Golgi complex to the nucleus. Proc Natl Acad Sci USA 96:4443-4448.
1999
10200281
2. Chang, K.H., Chen, Y., Chen, T.T., Chou, W.H., Chen, P.L., Ma, Y.Y., Yang-Feng, T.L., Leng, X., Tsai, M.J., O'Malley, B.W. and Lee, W-H. (1997) A thyroid hormone receptor coactivator negatively regulated by the retinoblastoma protein. Proc Natl Acad Sci USA 94:9040-9045.
1997
9256431
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