Product Name
XRCC4 antiserum (MS-XRC41-UP50)
Catalog Number
GTX70293
Product Description:
Mouse polyclonal antiserum to human XRCC4
Manufacturer & Mfg Part#:
GeneTex GTX70293
Application Note:
For WB: Use at a dilution of 1:1, 000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Background:
XRCC4 (X-ray cross complementation protein-4) is an essential component for DNA double strand break repair and V(D)J recombination. The XRCC4 gene encodes a 38kDa protein that is ubiquitously expressed and contains no sequence similarity to any previously described protein. Recent experiments have shown that XRCC4 directly interacts with DNA ligase IV implicating a possible role in DNA-end joining.
Target Protein MW:
46
Purity:
Mouse Polyclonal Antiserum
Concentration:
Antiserum
Storage Buffer:
Phosphate-buffered saline, pH 7.4, containing no preservatives
Cross-Reactivity:
Human - Not yet tested in other species
Target Protein:
XRCC4
Purification Note:
From ascitic fluid
Clonality:
Polyclonal
Tested Applications:
Western blot
Positive Control:
HeLa
Form:
Liquid
Isotype:
IgG
Immunogen:
Recombinant protein encoding full-length XRCC4 purified from E. coli.
Host:
Mouse
Storage:
Store at -80 °C. Avoid repeat freeze-thaw cycles.
General References:
1. Lieber, M.R. (1999) The biochemistry and biological significance of nonhomologous DNA end joining: an essential repair process in multicellular eukaryotes. Genes Cells:77-85.
1999
10320474
2. Bryans, M., Valenzano, M.C., Stamato, T.D. (1999) Absence of DNA ligase IV protein in XR-1 cells: evidence for stabilization by XRCC4. Mutat. Res. 433:53-58.
1999
10047779
3. Li, Z., Otevrel, T., Gao, Y., Cheng, H.L., Seed, B., Stamato, T.D., Taccioli, G.E., Alt, F.W. (1995) The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)Jrecombination. Cell 83: 1079-1089.
1995
8548796
4. Jeggo, P.A. (1998) Identification of genes involved in repair of DNA double-strand breaks in mammalian cells. Radiat. Res. 50:S80-91.
1998
980661
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Submitted by: User Review
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on 12/17/04
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We have used this Ab in immunofluorescence experiments on human cells fixed with PAF and permeabilized with triton. As expected, the Ab stains the nuclei and not the nucleoli (dilution 1/50 in pBS:BSA, 30 min incubation at room temperature). This Ab is less efficient as the rabbit polyclonal GTX20145 (dilution 1/300). However, it is useful in double-staining experiments with an other rabbit primary Ab
Dilution notes:
1/50 in PBS:BSA, at least 30 min at room temperature
Successful applications
Immunofluorescence |
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