Product Name
PCNA Interdomain Connector Loop antibody
Catalog Number
GTX70297
Product Description:
Mouse polyclonal to PCNA
Manufacturer & Mfg Part#:
GeneTex GTX70297
Application Note:
For WB: Use at a dilution of 1:1,000. Predicted molecular weight. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Background:
PCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor.for DNA polymerase d. It has also been shown to interact with several other proteins that are involved in DNA replication and repair, including replication factor C, DNA ligase I, XPG, FEN1 and p21. This partiular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur.
Immunogen:
Recombinant PCNA interdomain connector loop protein
Epitope:
Interdomain connector loop
Cross Reactivity:
Human - Not yet tested in other species
Tested Applications:
Western blot. The usefulness of this product in other applications has not been determined.
Target Protein:
PCNA
Cellular Localization:
Nuclear
Host:
Mouse
Clonality:
Polyclonal
Storage:
For long term storage, please keep at -70°C or below. Avoid repeared freeze-thaw cycles.
Purity :
Unpurified
Form:
Liquid
Purification Note:
Unpurified polyclonal serum.
Concentration:
Antiserum
Storage Buffer:
Phosphate-buffered saline, pH 7.2, containing no preservatives
Storage Instruction:
For long term storage, please keep at -70 °C or below. Avoid repeared freeze-thaw cycles.
General References:
1. Levin, DS et al. (2000). Interaction between PCNA and DNA ligase I is critical for joining of Okazaki fragments and long-patch base excision repair. Current Biol., 10:919-922.
2000
10959839
2. Warbrick, E et al. (1997). Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair. Oncogene 14, 2313-2321.
1997
9178907
3. Warbrick, E. (1998). PCNA binding through a conserved motif. Bioessays 20, 195-199.
1998
9631646
|
| Please tell us about your experience or submit a specific inquiry about this product. |
