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1 .GTX102287 eEF2 antibody [N2C1], Internal

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eEF2 antibody [N2C1], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX102287
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-eEF2 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102287
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
  • Anti-eEF2 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102287
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
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  • Application Information: eEF2 antibody [N2C1], Internal

Catalog Number GTX102287
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Product Name eEF2 antibody [N2C1], Internal
See all eEF2 products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size95 kDa (note)
Positive Controls293T , A431 , Neuro2A , NIH-3T3 , Neuro 2A , Raw264.7 , PC-12
Form SuppliedLiquid
Concentration0.86 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: eEF2 antibody [N2C1], Internal

Full Nameeukaryotic translation elongation factor 2
BackgroundThis gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq]
SynonymsEEF-2 antibody, EF2 antibody, EEF2 antibody, polypeptidyl-tRNA translocase antibody, elongation factor 2 antibody, eukaryotic translation elongation factor 2 antibody
Cellular LocalizationCytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human eEF2. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteChicken (95%), Rhesus Monkey (99%), Bovine (98%)
ConjugationUnconjugated

  • Storage Conditions: eEF2 antibody [N2C1], Internal

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
eEF2 antibody [N2C1], Internal validated data
  • Anti-eEF2 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102287
    • GTX102287 IHC-P Image
      eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis.
      Sample: Paraffin-embedded colon.
      eEF2 antibody (GTX102287) dilution: 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Sample(30 μg of whole cell lysate)
      A:293T
      B:A431(GTX27909)
      7.5% SDS PAGE
      GTX102287 diluted at 1:1500
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102287
    • GTX102287 IHC-P Image
      eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse intestine.
      eEF2 antibody (GTX102287) dilution: 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:1000.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102287
    • GTX102287 IHC-P Image
      eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis.
      Sample: Paraffin-embedded colon.
      eEF2 antibody (GTX102287) dilution: 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Sample(30 μg of whole cell lysate)
      A:293T
      B:A431(GTX27909)
      7.5% SDS PAGE
      GTX102287 diluted at 1:1500
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102287
    • GTX102287 IHC-P Image
      eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse intestine.
      eEF2 antibody (GTX102287) dilution: 1:500.
      Top
  • Anti-eEF2 antibody [N2C1], Internal used in Western Blot (WB). GTX102287
    • GTX102287 WB Image
      Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with eEF2 antibody [N2C1], Internal (GTX102287) diluted at 1:1000.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensEEF21938P13639, B2RMP5130610NM_001961NP_00195295.35

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusEef213629P58252NM_007907NP_03193395.33
Rattus norvegicusEef229565P05197NM_017245NP_05894195.3
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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