eIF2 alpha antibody [N1C1]
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||36 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa|
|Product Description||Rabbit Polyclonal antibody to eIF2 alpha (eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa)|
|Background||The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]|
|Synonyms||EIF-2 antibody, EIF-2A antibody, EIF-2alpha antibody, EIF2 antibody, EIF2A antibody, EIF2S1 antibody, eukaryotic translation initiation factor 2 subunit 1 antibody, eukaryotic translation initiation factor 2 subunit alpha antibody, eIF-2-alpha antibody, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (100%), Rat (100%), Xenopus laevis (94%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)|