|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||49 kDa (note)
|Positive Controls||Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||eukaryotic translation termination factor 1|
|Product Description||Rabbit Polyclonal antibody to eRF1 (eukaryotic translation termination factor 1)|
|Background||Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]|
|Synonyms||D5S1995 antibody, ERF antibody, ERF1 antibody, MGC111066 antibody, RF1 antibody, SUP45L1 antibody, TB3-1 antibody, ETF1 antibody, eukaryotic release factor 1 antibody, eukaryotic peptide chain release factor subunit 1 antibody, polypeptide chain release factor 1 antibody, protein Cl1 antibody, sup45 (yeast omnipotent suppressor 45) homolog-like 1 antibody, eukaryotic translation termination factor 1 antibody|
|Cellular Localization||Cytoplasm |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human eRF1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Rabbit, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Dog (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%)|