| Catalog Number | GTX70185 |
| Product Name | FEN1 antibody [4E7]
see all 'FEN1' products |
| Full Name | flap structure-specific endonuclease 1 |
| Synonyms | | RAD2 | P39748 | FEN-1 | 600393 | MF1 | FEN1 | 2237 | FEN 1 | |
| Product Description | Mouse monoclonal [4E7] FEN1 |
| Background | FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 4E7 |
| Subclass | IgG1 |
| Immunogen | Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli. |
| Antigen Species | Human |
| Species Reactivity | Human |
| Applications | ICC/IF, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 43 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Positive Controls | MCF7 |
| Target | FEN1 |
| Predicted Target Size(KDa) | 42 (Note) |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Protein G Affinity Purified |
| Purification Note | From hybridoma tissue culture supernatant |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Notes | For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption. |