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1 .GTX106208 hnRNP A1 antibody

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hnRNP A1 antibody

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 1 )
Cat No: GTX106208
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
  • Anti-hnRNP A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106208
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
  • Anti-hnRNP A1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX106208
  • Anti-hnRNP A1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX106208
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  • Bio-info
  • Protocol & MSDS
  • Application Information: hnRNP A1 antibody

Catalog Number GTX106208
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( 0 )
Publication ( 1 )
Product Name hnRNP A1 antibody
See all hnRNP A1 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size39 kDa (note) IsoForm (2)
Positive Controls293T , H1299 , HeLaS3 , mouse brain , PC-12
Form SuppliedLiquid
Concentration0.46 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: hnRNP A1 antibody

Full Nameheterogeneous nuclear ribonucleoprotein A1
BackgroundThis gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq]
SynonymsHNRPA1 antibody, MGC102835 antibody, hnRNP A1 antibody, hnRNP-A1 antibody, HNRNPA1 antibody, hnRNP core protein A1-like 3 antibody, hnRNP A1-like 3 antibody, single-strand RNA-binding protein antibody, heterogeneous nuclear ribonucleoprotein A1 antibody, Putative heterogeneous nuclear ribonucleoprotein A1-like 3 antibody, single-strand DNA-binding protein UP1 antibody, heterogeneous nuclear ribonucleoprotein B2 protein antibody, helix-destabilizing protein antibody, heterogeneous nuclear ribonucleoprotein A1B protein antibody, nuclear ribonucleoprotein particle A1 protein antibody, heterogeneous nuclear ribonucleoprotein core protein A1 antibody, hnRNP core protein A1 antibody
Cellular LocalizationNucleus , Cytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human hnRNP A1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, Bovine
Predict Reactivity NoteXenopus laevis (92%), Pig (100%), Chicken (97%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: hnRNP A1 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
hnRNP A1 antibody validated data
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody (GTX106208) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-hnRNP A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106208
    • GTX106208 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1(GTX106208) antibody at 1:200 dilution.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      Sample(30 µg of whole cell lysate)
      A:H1299
      B:HeLa S3(GTX14654)
      12% SDS PAGE
      GTX106208 diluted at 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX106208 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      hnRNP A1 antibody detects HNRNPA1 protein by western blot analysis.
      A. 30 µg PC-12 whole cell lysate/extract
      12% SDS-PAGE
      hnRNP A1 antibody (GTX106208) dilution: 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-hnRNP A1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX106208
    • GTX106208 IHC-P Image
      hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fore brain.
      hnRNP A1 antibody (GTX106208) diluted at 1:500.
      Top
  • Anti-hnRNP A1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX106208
    • GTX106208 IHC-P Image
      hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse heart.
      hnRNP A1 antibody (GTX106208) diluted at 1:500.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody (GTX106208) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-hnRNP A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106208
    • GTX106208 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP A1(GTX106208) antibody at 1:200 dilution.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      Sample(30 µg of whole cell lysate)
      A:H1299
      B:HeLa S3(GTX14654)
      12% SDS PAGE
      GTX106208 diluted at 1:500
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX106208 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-hnRNP A1 antibody used in Western Blot (WB). GTX106208
    • GTX106208 WB Image
      hnRNP A1 antibody detects HNRNPA1 protein by western blot analysis.
      A. 30 µg PC-12 whole cell lysate/extract
      12% SDS-PAGE
      hnRNP A1 antibody (GTX106208) dilution: 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-hnRNP A1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX106208
    • GTX106208 IHC-P Image
      hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fore brain.
      hnRNP A1 antibody (GTX106208) diluted at 1:500.
      Top
  • Anti-hnRNP A1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX106208
    • GTX106208 IHC-P Image
      hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse heart.
      hnRNP A1 antibody (GTX106208) diluted at 1:500.
      Top
Back to Top
  • Reference for hnRNP A1 antibody
Application References Application Reactivity
Weng KF et al. A cytoplasmic RNA virus generates functional viral small RNAs and regulates viral IRES activity in mammalian cells. Nucleic Acids Res 42 (20):12789-805 (2014).
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensHNRNPA13178P09651, A8K4Z8164017NM_002136NP_00212734.2
Homo sapiensHNRNPA13178P09651, A8K4Z8164017NM_031157NP_11242038.75

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusHnrnpa115382P49312, Q5EBP8NM_001039129NP_00103421838.84
Mus musculusHnrnpa115382P49312, Q5EBP8NM_010447NP_03457734.2
Rattus norvegicusHnrnpa129578P04256NM_017248NP_05894434.22
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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