hnRNP F antibody [N1N3]
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||46 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||heterogeneous nuclear ribonucleoprotein F|
|Product Description||Rabbit polyclonal antibody to hnRNP F (heterogeneous nuclear ribonucleoprotein F)|
|Background||This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]|
|Synonyms||HNRPF antibody, MGC110997 antibody, OK/SW-cl.23 antibody, mcs94-1 antibody, HNRNPF antibody, HnRNP F protein antibody, heterogeneous nuclear ribonucleoprotein F antibody, nucleolin-like protein mcs94-1 antibody|
|Cellular Localization||Nucleus , nucleoplasm |
|Specificity||Knockdown/Knockout validation was supported by references (PMID:26991008)|
|Target||hnRNP F |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human hnRNP F. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%)|