KPNA2 antibody [C3], C-term
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||58 kDa (note)
|Positive Controls||293T , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||karyopherin alpha 2 (RAG cohort 1, importin alpha 1)|
|Product Description||Rabbit polyclonal antibody to KPNA2 (karyopherin alpha 2 (RAG cohort 1, importin alpha 1))|
|Background||The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq]|
|Synonyms||IPOA1 antibody, QIP2 antibody, RCH1 antibody, SRP1alpha antibody, KPNA2 antibody, karyopherin subunit alpha-2 antibody, SRP1-alpha antibody, RAG cohort 1 antibody, importin alpha 1 antibody, importin subunit alpha-2 antibody, RAG cohort protein 1 antibody, pendulin antibody, importin-alpha-P1 antibody, importin alpha 2 antibody, "karyopherin alpha 2 (RAG cohort 1, importin alpha 1) antibody"|
|Cellular Localization||Cytoplasm , Nucleus |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human KPNA2. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Chimpanzee|
|Predict Reactivity Note||Mouse (100%), Rat (100%), Chimpanzee (100%)|