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1 .GTX128508 mCherry antibody

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mCherry antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-Fr, IP, WB, IHC-Wm
  • Publication  ( 3 )
Cat No: GTX128508
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-mCherry antibody used in IHC (Whole mount). GTX128508
  • Anti-mCherry antibody used in Western blot. GTX128508
  • Anti-mCherry antibody used in Immunoprecipitation. GTX128508
  • Anti-mCherry antibody used in Western blot. GTX128508
  • Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence. GTX128508
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  • Datasheet
  • Star Review (0)
    Reference (3)
  • Protocol & MSDS
  • Application Information: mCherry antibody

Catalog Number GTX128508
☆☆☆☆☆
( 0 )
Publication ( 3 )
Product Name mCherry antibody
See all mCherry products
ApplicationsICC/IF, IHC-Fr, IP, WB, IHC-Wm
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence1:100-1:1000*  
IHC (Frozen sections)Assay-dependent dilution Y
Immunoprecipitation1:100-1:500*  
Western blot1:1000-1:10000* Y
IHC (Whole mount)1:100-1:500*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size27 kDa (note)
Positive Controlshuman PLK4-mCherry-transfected 293T
Form SuppliedLiquid
Concentration0.36 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: mCherry antibody

Product DescriptionRabbit Polyclonal antibody to mCherry
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated

  • Storage Conditions: mCherry antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
mCherry antibody validated data
  • Anti-mCherry antibody used in IHC (Whole mount). GTX128508
    • GTX128508 IHC-Wm Image
      mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
      Sample: 2 days-post-fertilization zebrafish embryo.
      mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.
      Top
  • Anti-mCherry antibody used in Western blot. GTX128508
    • GTX128508 WB Image
      mCherry antibody detects mCherry protein by western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-mCherry antibody used in Immunoprecipitation. GTX128508
    • GTX128508 IP Image
      mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128508) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-mCherry antibody used in Western blot. GTX128508
    • GTX128508 WB Image
      mCherry antibody detects mCherry (full length) protein by western blot analysis.
      A. 5ng recombinant mCherry
      B. 10ng recombinant mCherry
      C. 20ng recombinant mCherry
      12% SDS-PAGE
      mCherry antibody (GTX128508) dilution: 1:2000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence. GTX128508
    • GTX128508 ICC/IF Image
      mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis.
      Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min.
      Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
      Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.
      Top
  • Anti-mCherry antibody used in IHC (Whole mount). GTX128508
    • GTX128508 IHC-Wm Image
      mCherry antibody labels mCherry-expressing cells on zebrafish embryos by whole mount immunohistochemical analysis.
      Sample: 2 days-post-fertilization zebrafish embryo.
      mCherry antibody labels mCherry-expressing cells (GTX128508) dilution: 1:100.
      Top
  • Anti-mCherry antibody used in Western blot. GTX128508
    • GTX128508 WB Image
      mCherry antibody detects mCherry protein by western blot analysis. Non-transfected (-) and PLK4 -transfected (+, inculding mCherry -tag) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mCherry antibody (GTX128508) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-mCherry antibody used in Immunoprecipitation. GTX128508
    • GTX128508 IP Image
      mcherry antibody immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of mcherry protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of mcherry protein by 2.5 μg of mcherry antibody (GTX128508) 7.5% SDS-PAGE The immunoprecipitated mcherry protein was detected by mcherry antibody (GTX128508) diluted at 1 : 3000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-mCherry antibody used in Western blot. GTX128508
    • GTX128508 WB Image
      mCherry antibody detects mCherry (full length) protein by western blot analysis.
      A. 5ng recombinant mCherry
      B. 10ng recombinant mCherry
      C. 20ng recombinant mCherry
      12% SDS-PAGE
      mCherry antibody (GTX128508) dilution: 1:2000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-mCherry antibody used in Immunocytochemistry/ Immunofluorescence. GTX128508
    • GTX128508 ICC/IF Image
      mCherry antibody detects mCherry protein at mCherry fusion protien by immunofluorescent analysis.
      Sample: mCherry fusion protien-transfected HeLa cells were fixed in Triton X-100 for 1 min, then 4% paraformaldehyde at RT for 30 min.
      Green: mCherry protein stained by mCherry antibody (GTX128508) diluted at 1:500.
      Red: mCherry is expressed in the tranfected cell . Blue: Hoechst 33342 staining.
      Top
Back to Top
  • Reference for mCherry antibody
Application References Application Reactivity
Yao X et al. Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res 27 (6):801-814 (2017). IHC-Fr
Yang FM et al. A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Biochem J 471 (2):199-209 (2015). WB
Chou CM et al. Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish. J Biomed Sci 22:102 (2015). WB
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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