mTOR antibody [C3], C-term
|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||289 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||mechanistic target of rapamycin (serine/threonine kinase)|
|Product Description||Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))|
|Background||The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]|
|Synonyms||FLJ44809 antibody, FRAP antibody, FRAP1 antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, MTOR antibody, FK506 binding protein 12-rapamycin associated protein 2 antibody, FK506-binding protein 12-rapamycin complex-associated protein 1 antibody, FKBP-rapamycin associated protein antibody, mammalian target of rapamycin antibody, rapamycin associated protein FRAP2 antibody, FKBP12-rapamycin complex-associated protein 1 antibody, serine/threonine-protein kinase mTOR antibody, FK506 binding protein 12-rapamycin associated protein 1 antibody, rapamycin and FKBP12 target 1 antibody, rapamycin target protein 1 antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody|
|Cellular Localization||Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Zebrafish (90%), Sheep (98%)|