p27 Kip1 antibody
|Applications||ICC/IF, IHC-Fr, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||22 kDa (note)
|Positive Controls||MCF-7 , NIH-3T3 , CCRF-SB , MFB (mouse fibroblast) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||cyclin-dependent kinase inhibitor 1B (p27, Kip1)|
|Product Description||Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))|
|Background||This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq]|
|Synonyms||CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, CDKN1B antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody|
|Cellular Localization||Nucleus , Cytoplasm|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Cat, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%)|