p27 Kip1 antibody, Internal
|Applications||IHC, IHC-P, WB|
|Application Note||Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xia et al, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14028-33; PMID: 16169901). Recommended concentration: 0.1-0.3µg/ml. |
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX89392-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||cyclin-dependent kinase inhibitor 1B (p27, Kip1)|
|Product Description||Goat polyclonal antibody to KIP1 / CDKN1B (internal)|
|Background||Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events. |
|Synonyms||P27KIP1, CDKN1B, cyclindependent kinase inhibitor 1B (p27, Kip1), cyclindependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), cyclindependent kinase inhibitor1B (p27, Kip1), KIP1, KIP1 / CDKN1B (internal), cyclindependent kinase inhibitor1B, cyclin-dependent kinase inhibitor 1B, CDKN4, MEN1B, MEN4|
|Cellular Localization||Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation t|
|Specificity||This antibody is specific for KIP1 / CDKN1B (internal).|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Cat, Dog, Pig, Bovine|