|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||44 kDa (note)
|Positive Controls||293T , A431 , HCT116 , HCT116 24hr cisplatin 30uM , *HUVEC|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||tumor protein p53|
|Product Description||Rabbit Polyclonal antibody to p53 (tumor protein p53)|
|Background||This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]|
|Synonyms||FLJ92943 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, TP53 antibody, cellular tumor antigen p53 antibody, antigen NY-CO-13 antibody, phosphoprotein p53 antibody, p53 tumor suppressor antibody, transformation-related protein 53 antibody, tumor protein p53 antibody|
|Cellular Localization||Cytoplasm , Nucleus , Endoplasmic reticulum |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Dog, Cat, Pig, Rabbit, Sheep, Rhesus Monkey, Bovine, Guinea pig|
|Predict Reactivity Note||Rat (86%), Dog (91%), Cat (90%), Pig (89%), Rabbit (92%), Sheep (86%), Rhesus Monkey (97%), Bovine (86%), Guinea pig (83%)|