|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||54 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||non-POU domain containing, octamer-binding|
|Product Description||Rabbit polyclonal antibody to p54/nrb (non-POU domain containing, octamer-binding)|
|Background||This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq]|
|Synonyms||NMT55 antibody, NRB54 antibody, P54 antibody, P54NRB antibody, NONO antibody, "DNA-binding p52/p100 complex, 52 kDa subunit antibody", non-POU domain-containing octamer (ATGCAAAT) binding protein antibody, p54(nrb) antibody, 55 kDa nuclear protein antibody, non-POU domain-containing octamer-binding protein antibody, 54 kDa nuclear RNA- and DNA-binding protein antibody, "non-POU domain containing, octamer-binding antibody"|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human p54/nrb. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (100%), Xenopus laevis (86%), Chicken (98%), Chimpanzee (100%), Bovine (99%)|