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Catalog Number
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GTX46123
(
0
)
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Product Name
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p73 antibody [5B429]
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| Applications | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB, ChIP assay, MS |
| Application Note |
| Suggested dilution | Reference |
| ChIP assay | 1:20 - 1:1000* |
|
| Flow cytometry/FACS | 1 μg/ml* |
|
| Immunocytochemistry/ Immunofluorescence (ICC/IF) | 1:20 - 1:1000* |
|
| IHC (Frozen sections) | 5 - 15 μg/ml* |
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| IHC (Formalin-fixed paraffin-embedded sections) | 5 - 15 μg/ml* |
|
| Immunoprecipitation | 1:20 - 1:1000* |
|
| Western Blot (WB) | 2 μg/ml* |
|
| Mass spectrometry | Assay-dependent dilution |
|
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 70 (note)
|
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Protein G purified |
| Full Name | tumor protein p73 |
| Background | This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq] |
| Synonyms | P73 antibody |
| Cellular Localization | Nucleus |
| Specificity | p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 antibody (clone 5B429) reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Name | 5B429 |
| Isotype | IgG1 |
| Immunogen | This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53. |
| Light Chain | Kappa |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |