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p73 antibody [N1C1]

Anti-p73 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109045
Anti-p73 antibody [N1C1] used in Western Blot (WB). GTX109045
Anti-p73 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109045
Anti-p73 antibody [N1C1] used in Western Blot (WB). GTX109045
Anti-p73 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109045
Anti-p73 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109045

Cat. No. GTX109045

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p73. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952351

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p73 , P73

Cellular Localization

Nucleus

Background

This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p73 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109045

GTX109045 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using p73 (GTX109045) antibody at 1:100 dilution.

Anti-p73 antibody [N1C1] used in Western Blot (WB). GTX109045

GTX109045 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p73 antibody [N1C1] (GTX109045) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p73 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109045

GTX109045 IHC-P Image

p73 antibody [N1C1] detects p73 protein at nucleus in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
p73 antibody [N1C1] (GTX109045) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p73 antibody [N1C1] used in Western Blot (WB). GTX109045

GTX109045 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p73 antibody [N1C1] (GTX109045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p73 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109045

GTX109045 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p73 (GTX109045) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p73 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX109045

GTX109045 IHC-P Image

p73 antibody [N1C1] detects p73 protein at nucleus in human SCC xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human SCC xenograft .
p73 antibody [N1C1] (GTX109045) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169