| Catalog Number | GTX70220 |
| Product Name | p84 antibody [5E10] |
| Full Name | THO complex 1 |
| Synonyms | | HPR1 | P84 | P84N5 | |
| Product Description | Mouse monoclonal [5E10] to human p84 |
| Background | P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 5E10 |
| Subclass | IgG2b |
| Immunogen | Amino acids 15-374 of human p84 expressed in E. coli. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Rat |
| Applications | ICC/IF, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | Recommended Starting Dilutions:
For IF: Use at a concentration of 0.5 - 2 µg/ml.
For IP: Use at a concentration of 0.5 - 2 µg/ml.
For WB: Use at a concentration of 0.3 - 2 µg/ml.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Positive Controls | HeLa | Raji | MOLT4 | 293T | NIH-3T3 | mouse brain | PC-12 | HeLa nucleus | Jurkat | A431 | HepG2 | H1299 | HCT116 | MCF-7 | NT2D1 | PC-3 | U87-MG |
| Target | p84 |
| Predicted Target Size(KDa) | 84 kDa (Note) |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Protein G Affinity Purified |
| Purification Note | From ascitic fluid |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Notes | For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption. |