|Product Name||p84 antibody [5E10]|
|Full Name||THO complex 1|
|Synonyms||| HPR1 | P84 | P84N5 ||
|Product Description||Mouse monoclonal [5E10] to human p84|
|Background||P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies. |
|Immunogen||Amino acids 15-374 of human p84 expressed in E. coli.|
|Species Reactivity||Human, Mouse, Rat|
|Applications||ICC/IF, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined.|
|Application Note||Recommended Starting Dilutions:|
For IF: Use at a concentration of 0.5 - 2 µg/ml.
For IP: Use at a concentration of 0.5 - 2 µg/ml.
For WB: Use at a concentration of 0.3 - 2 µg/ml.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||HeLa | Raji | MOLT4 | 293T | NIH-3T3 | mouse brain | PC-12 | HeLa nucleus | Jurkat | A431 | HepG2 | H1299 | HCT116 | MCF-7 | NT2D1 | PC-3 | U87-MG|
|Predicted Target Size(KDa) ||84 kDa (Note)|
|Purification||Protein G Affinity Purified|
|Purification Note||From ascitic fluid|
|Storage Buffer||Phosphate-buffered saline, pH 7.4, containing no preservatives |
|Storage Instruction||Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.|
|Notes||For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption.|